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KMID : 1094719990040010072
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 1 p.72 ~ p.72
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Regiospecific orientation of single-chain antibody and atomic force microscope (AFM) images
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Yun Kyu-Sik
Park Seon-Hee
Pyo Hyeon-Bong
Kim Seung-Hwan
Lee Soo-Yeul
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Abstract
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An antibody containing a genetically engineered lipid group at the N-terminus and a hexahistidinyl tag at the C-terminus (Lpp-scFv-His6) was immobilized in an oriented manner on the surface of liposomes. Liposomes, consisting of antibody and phosphatidylcholine, have been prepared and imaged by AFM. For AFM visualization, the resulting liposomes were bound on the surface of mica by two different mechanisms. The histidine tags present in the antibody molecules of the immunoliposome were anchored to the NiCl2 treated mica surface. Alternatively, the immunoliposomes were immunochemically bound on antigen-coated mica surface. Both approaches yielded liposomes which were clearly imaged without damage by AFM in ambient condition.
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KEYWORD
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lipid tagged single chain antibody(Lpp-scFv), AFM, antigen, antibody
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FullTexts / Linksout information
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